EXPRESSION OF A YEAST INTRON-CONTAINING TRANSFER-RNA IN THE ARCHAEON HALOFERAX-VOLCANII

被引：20

作者：

PALMER, JR ^{[1
]}

NIEUWLANDT, DT ^{[1
]}

DANIELS, CJ ^{[1
]}

机构：

[1] OHIO STATE UNIV,DEPT MICROBIOL,COLUMBUS,OH 43210

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 12期

关键词：

D O I：

10.1128/JB.176.12.3820-3823.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Expression of the yeast tRNAPro(UGG) gene in Haloferax volcanii resulted in the production of a single stable transcript that had not undergone intron processing or processing of 5' and 3' flanking sequences. Mutation of the exon-intron boundary region of this RNA to produce a precursor RNA with the preferred halobacterial consensus exon-intron boundary structure did not restore intron processing; Processing of 5' and 3' flanking sequences was restored when the acceptor stem U6-U67 pair was changed to A6-U67. The significance of these results in defining the recognition requirements of tRNA maturation enzymes in the halolphilic domain Archaea is discussed.

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页码：3820 / 3823

页数：4

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