TCE DEGRADATION IN A METHANOTROPHIC ATTACHED-FILM BIOREACTOR

被引:47
作者
FENNELL, DE [1 ]
NELSON, YM [1 ]
UNDERHILL, SE [1 ]
WHITE, TE [1 ]
JEWELL, WJ [1 ]
机构
[1] CORNELL UNIV, DEPT AGR & BIOL ENGN, ITHACA, NY 14853 USA
关键词
METHANOTROPH; TRICHLOROETHANE; EXPANDED BED; ATTACHED FILM; BIODEGRADATION;
D O I
10.1002/bit.260420711
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Trichloroethene was degraded in expanded-bed bioreactors operated with mixed-culture methanotrophic attached films. Biomass concentrations of 8 to 75 g volatile solids (VS) per liter static bed (L(sb)) were observed. Batch TCE degradation rates at 35-degrees-C followed the Michaelis-Menten model, and a maximum TCE degradation rate (q(max)) of 10.6 mg TCE/gVS . day and a half velocity coefficient (K(S) of 2.8 mg TCE/L were predicted. Continuous-flow kinetics also followed the Michaelis-Menten model, but other parameters may be limiting, such as dissolved copper and dissolved methane-q(max) and K(S) were 2.9 mg TCE/gVS . day and 1.5 mg TCE/L, respectively, at low copper concentrations (0.003 to 0.006 mg Cu/L). The maximum rates decreased substantially with small increases in dissolved copper. Methane consumption during continuous-flow operation varied from 23 to 1200 g CH4/g TCE degraded. Increasing the influent dissolved methane concentration from 0.01 mg/L to 5.4 mg/L reduced the TCE degradation rate by nearly an order of magnitude at 21-degrees-C. Exposure of biofilms to 1.4 mg/L tetrachloroethene (PCE) at 35-degrees-C resulted in the loss of methane utilization ability. Tests with methanotrophs grown on granular activated carbon indicated that lower effluent TCE concentrations could be obtained. The low efficiencies of TCE removal and low degradation rates obtained at 35-degrees-C suggest that additional improvements will be necessary to make methanotrophic TCE treatment attractive. (C) 1993 John Wiley & Sons, Inc.
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收藏
页码:859 / 872
页数:14
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