PURIFICATION AND CHARACTERIZATION OF A HEAT-STABLE ENTEROTOXIN OF VIBRIO-MIMICUS

被引：21

作者：

ARITA, M

HONDA, T

MIWATANI, T

TAKEDA, T

TAKAO, T

SHIMONISHI, Y

机构：

[1] OSAKA UNIV, MICROBIAL DIS RES INST, 3-1 YAMADAOKA, SUITA, OSAKA 565, JAPAN

[2] OSAKA UNIV, INST PROT RES, SUITA, OSAKA 565, JAPAN

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 79卷 / 01期

关键词：

HEAT-STABLE ENTEROTOXIN; VIBRIO-MIMICUS; VIBRIO-CHOLERAE NON-O1;

D O I：

10.1016/0378-1097(91)90536-J

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A heat-stable enterotoxin produced by Vibrio mimicus (VM-ST) was studied. VM-ST was purified from a culture supernatant of V. mimicus strain AQ-0915 by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatography on both SP-Sephadex C-50 and DEAE-Sephadex A-25, and HPLC, and the recovery rate was about 15%. Purified VM-ST was heat-stable. VM-ST activity was cross-neutralized by anti-STh antiserum. The amino acid composition of the purified VM-ST was determined 17 amino acid residues in the following sequence: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This composition and sequence were identical to those of V. cholerae non-O1-ST. These results clearly demonstrate the production of a characteristic VM-ST by V. mimicus.

引用

页码：105 / 110

页数：6

共 16 条

[11] PURIFICATION OF ENTEROTOXINS FROM VIBRIO-MIMICUS THAT APPEAR TO BE IDENTICAL TO CHOLERA-TOXIN [J].