APPEARANCE AND LOCALIZATION OF A BETA-GLUCOSIDASE HYDROLYZING CONIFERIN IN SPRUCE (PICEA-ABIES) SEEDLINGS

被引：32

作者：

MARCINOWSKI, S ^{[1
]}

FALK, H ^{[1
]}

HAMMER, DK ^{[1
]}

HOYER, B ^{[1
]}

GRISEBACH, H ^{[1
]}

机构：

[1] MAX PLANCK INST IMMUNBIOL, D-7800 FREIBURG, FED REP GER

来源：

PLANTA | 1979年 / 144卷 / 02期

关键词：

β-Glucosidase; Coniferin; Immunofluorescent studies; Lignification; Picea;

D O I：

10.1007/BF00387265

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

.beta.-Glucosidase activity for coniferin (coniferyl alcohol .beta.-D-glucoside) is not present in spruce (Picea abies L. Karst.) seeds but appears in the young seedlings. Lignification starts at approximately day 9 of germination in the vascular bundles. An antiserum against glucosidase 1 isolated from spruce seedlings was employed for the localization of the enzyme in cross sections of hypocotyls using immunofluorescent technique. Apparently at this stage of development the glucosidase is localized at the inner layer of the secondary cell wall. Glucosidase activity was present in all cells of the investigated hypocotyl tissue.

引用

页码：161 / 165

页数：5

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