CONJUGATES OF FLUORESCEIN AND SAENTA (5'-S-(2-AMINOETHYL)-N-6-(4-NITROBENZYL)-5'-THIOADENOSINE) - FLOW-CYTOMETRY PROBES FOR THE ES NUCLEOSIDE TRANSPORTER ELEMENTS OF THE PLASMA-MEMBRANE

被引:24
作者
BUOLAMWINI, JK
CRAIK, JD
WILEY, JS
ROBINS, MJ
GATI, WP
CASS, CE
PATERSON, ARP
机构
[1] UNIV ALBERTA,DEPT PHARMACOL,EDMONTON T6G 2H7,AB,CANADA
[2] UNIV ALBERTA,DEPT BIOCHEM,EDMONTON T6G 2H7,AB,CANADA
[3] AUSTIN HOSP,DEPT HAEMATOL,MELBOURNE,VIC 3084,AUSTRALIA
[4] BRIGHAM YOUNG UNIV,DEPT CHEM,PROVO,UT 84602
来源
NUCLEOSIDES & NUCLEOTIDES | 1994年 / 13卷 / 1-3期
基金
英国医学研究理事会;
关键词
D O I
10.1080/15257779408013276
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
SAENTA was linked to the C-5 or C-6 positions of fluorescein through several structures to form conjugates that were bound tightly to plasma membrane sites associated with es nucleoside transport activity. The conjugates imparted fluorescence to cells that expressed es nucleoside transport activity and served as es-selective plasma membrane stains suitable for flow cytometry. Prior treatment of es-expressing cells with nitrobenzylthioinosine prevented fluorescent staining with the conjugates. Seven SAENTA-fluorescein conjugates served as now cytometric stains with high affinities for es sites, despite substantial differences in the SAENTA-fluorescein linkage structures.
引用
收藏
页码:737 / 751
页数:15
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