PROTEIN-FREE HUMAN-HUMAN HYBRIDOMA CULTURES IN AN INTERCALATED-SPIRAL ALTERNATE-DEAD-ENDED HOLLOW-FIBER BIOREACTOR

被引：10

作者：

BROTHERTON, JD ^{[1
]}

CHAU, PC ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,DEPT AMES CHEM ENGN,LA JOLLA,CA 92093

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1995年 / 47卷 / 03期

关键词：

HOLLOW FIBER; BIOREACTOR; HYBRIDOMA;

D O I：

10.1002/bit.260470312

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Batch cell cultures of a human-human hybridoma line in a convective flow dominant intercalated-spiral alternate-dead-ended hollow fiber are compared with those using conventional axial-flow hollow fiber bioreactors and a stirred-tank bioreactor. Relatively short-term fed-batch and perfusion cell cultures were also employed for the intercalated-spiral bioreactor. When operating conditions of a batch intercalated-spiral bioreactor were properly chosen, the cell growth and substrate consumption paralleled that of a batch stirred-tank culture. The results verified the premise of the intercalated-spiral hollow fiber bioreactor that nutrient transport limitations can be eliminated when the convective flux through the extracapillary space is sufficiently high. (C) 1995 John Wiley and Sons, Inc.

引用

页码：384 / 400

页数：17

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