ISOLATION AND PURIFICATION OF 7S-GLOBULIN AND 11S-GLOBULIN FROM BROAD BEANS AND PEAS

被引：38

作者：

SUCHKOV, VV ^{[1
]}

POPELLO, IA ^{[1
]}

GRINBERG, VY ^{[1
]}

TOLSTOGUSOV, VB ^{[1
]}

机构：

[1] AN NESMEYANOV HETEROORGAN CPDS INST,VAVILOV ST 28,MOSCOW 117312,USSR

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1990年 / 38卷 / 01期

关键词：

D O I：

10.1021/jf00091a017

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A simple and effective preparation technique for isolation of 7S and 11S globulins from broad beans and pea seeds has been developed. It is based on differential solubility of 7S and 11S globulins in sodium chloride solution at pH 4.8 and at 5 and 20 °C. The method makes it possible to isolate 6 g of 11S and 1 g of 7S globulins from 100 g of broad bean meal or 1.5 g of US and 2 g of 7S globulins from 100 g of pea meal, with only low centrifugation. If necessary, the admixture of 15S component in 11S globulin preparations can be removed by gel chromatography on Sepharose CL-6B. © 1990, American Chemical Society. All rights reserved.

引用

页码：92 / 95

页数：4