THE USE OF STOKES RAMAN-SCATTERING IN TIME CORRELATED SINGLE PHOTON-COUNTING - APPLICATION TO THE FLUORESCENCE LIFETIME OF TYROSINATE

被引：18

作者：

WILLIS, KJ ^{[1
]}

SZABO, AG ^{[1
]}

KRAJCARSKI, DT ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,DIV BIOL SCI,OTTAWA K1A 0R6,ONTARIO,CANADA

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1990年 / 51卷 / 03期

关键词：

D O I：

10.1111/j.1751-1097.1990.tb01725.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— The decay kinetics of the tyrosinate form of tyrosine in aqueous solution, pH 11, were investigated by time correlated single photon counting using laser excitation. The lifetime of the single exponential decay was 30.5 ± 0.2 ps. Stokes Raman scattering was used to determine the instrument response function and the advantages of this approach over the conventional Rayleigh scattering method are discussed. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：375 / 377

页数：3

共 15 条

[11]

ROSS JBA, 1990, IN PRESS FLUORESCENC, V2

[12] ELIMINATION OF EXCESS PULSE BROADENING AT HIGH SPECTRAL RESOLUTION OF PICOSECOND DURATION LIGHT-EMISSION [J].

SAARI, P ;

AAVIKSOO, J ;

FREIBERG, A ;

TIMPMANN, K .

OPTICS COMMUNICATIONS, 1981, 39 (1-2) :94-98

[13] POSSIBILITIES AND LIMITATIONS OF THE TIME-CORRELATED SINGLE PHOTON-COUNTING TECHNIQUE - A COMPARATIVE-STUDY OF CORRECTION METHODS FOR THE WAVELENGTH DEPENDENCE OF THE INSTRUMENT RESPONSE FUNCTION [J].

VANDENZEGEL, M ;

BOENS, N ;

DAEMS, D ;

DESCHRYVER, FC .

CHEMICAL PHYSICS, 1986, 101 (02) :311-335

[14] MICROCHANNEL-PLATE PHOTOMULTIPLIER APPLICABILITY TO THE TIME-CORRELATED PHOTON-COUNTING METHOD [J].

YAMAZAKI, I ;

TAMAI, N ;

KUME, H ;

TSUCHIYA, H ;

OBA, K .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1985, 56 (06) :1187-1194

[15] DELTA FUNCTION CONVOLUTION METHOD (DFCM) FOR FLUORESCENCE DECAY EXPERIMENTS. [J].

Zuker, M. ;

Szabo, A.G. ;

Bramall, L. ;

Krajcarski, D.T. ;

Selinger, B. .

Review of Scientific Instruments, 1985, 56 (01) :14-22

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