ESTIMATION OF HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC RETENTION INDEXES

A method for the prediction of the retention times on C18 reverse phase columns is developed and applied to a series of propranolol, anthranillc acid, and barbiturate analogues. The retention properties of the drugs are measured using a retention index scale that is based on the relative retention of a series of 2-keto alkane standards. The retention index (I) of the test compounds is estimated using the equation I = 200π + Iref where Iref is the observed retention index of one reference compound that is structurally related to the other compounds and π is the sum of the Hansch substituent constants for the test compound. © 1979, American Chemical Society. All rights reserved.