DETECTION OF INTRACELLULAR INTERLEUKIN-10 BY FLOW-CYTOMETRY

被引：14

作者：

KREFT, B ^{[1
]}

SINGER, GG ^{[1
]}

DIAZGALLO, C ^{[1
]}

KELLEY, VR ^{[1
]}

机构：

[1] BRIGHAM & WOMENS HOSP,DEPT MED,DIV RENAL,TRANSPLANTAT & IMMUNOGENET LAB,MOLEC AUTOIMMUN SECT,BOSTON,MA 02115

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 156卷 / 01期

关键词：

INTERLEUKIN-10; INTRACELLULAR EXPRESSION; FLOW CYTOMETRY; DIGITONIN; T-CELL PERMEABILIZATION;

D O I：

10.1016/0022-1759(92)90018-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Detection of cytokines is limited to measurements of the secreted molecule. To circumvent this problem we permeabilized the cell membrane with digitonin and localize cytokine expression using antibodies by flow cytometry. In this report we demonstrate that we can detect specific intracellular interleukin-10 (IL-10) in the HT-2 T cell line only after membrane permeabilization. With this technique intracellular cytokines are readily detectable.

引用

页码：125 / 128

页数：4

共 12 条

[1]

AARGON JJ, 1980, P NATL ACAD SCI USA, V77, P6324

[2]

ANDERSON P, 1989, J IMMUNOL, V143, P1899

[3]

FIORENTINO DF, 1991, J IMMUNOL, V147, P3815

[4] THE CYTOSKELETON OF DIGITONIN-TREATED RAT HEPATOCYTES [J].

FISKUM, G ;

CRAIG, SW ;

DECKER, GL ;

LEHNINGER, AL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (06) :3430-3434

[5] PERMEABILIZATION OF PLATELETS - AN INVESTIGATION OF BIOCHEMICAL, ULTRASTRUCTURAL AND FUNCTIONAL-ASPECTS [J].

LINEBERGER, B ;

DAWICKI, DD ;

AGARWAL, KC ;

KESSIMIAN, N ;

STEINER, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1012 (01) :36-45

[6]

MALEFYT RD, 1991, J EXP MED, V174, P915

[7]

MATIS LA, 1983, J IMMUNOL, V130, P1527

[8] Homology of Cytokine Synthesis Inhibitory Factor (IL-10) to the Epstein-Barr Virus Gene BCRFI [J].

Moore, Kevin W. ;

Vieira, Paulo ;

Fiorentino, David F. ;

Trounstine, Mary L. ;

Khan, Tariq A. ;

Mosmann, Timothy R. .

JOURNAL OF IMMUNOLOGY, 2012, 189 (05) :1230-1234

[9]

MOSMANN T, 1991, CURRENT PROTOCOLS IM, V1

[10]

MOSMANN TR, 1990, J IMMUNOL, V145, P2938

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