C-13-NMR OF THE DEOXYRIBOSE SUGARS IN 4 DNA OLIGONUCLEOTIDE DUPLEXES - ASSIGNMENT AND STRUCTURAL FEATURES

被引：18

作者：

LAPLANTE, SR ^{[1
]}

ZANATTA, N ^{[1
]}

HAKKINEN, A ^{[1
]}

WANG, AHJ ^{[1
]}

BORER, PN ^{[1
]}

机构：

[1] SYRACUSE UNIV,CTR SCI & TECHNOL,DEPT CHEM,SYRACUSE,NY 13244

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 09期

关键词：

D O I：

10.1021/bi00175a011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Natural-abundance C-13-NMR spectra have been obtained for four self-complementary DNA oligonucleotides: [d(TAGCGCTA)](2), [d(GGTATACC)](2), [d(CG)(3)](2), and [d(TCGCG)](2); this paper focuses on the deoxyribose resonances. Assignments were made by a combination of the two-dimensional proton-detected heteronuclear correlation experiment and comparison of 1D spectra, accounting for P-31 coupling, bask composition, and similarities in chemical shift versus temperature profiles (delta vs T). Large shielding and deshielding of the sugar resonances (between 2.0 and -1.9 ppm) are observed upon thermal dissociation of the duplex. The shapes of the delta vs T profiles correlate strongly with the purine/pyrimidine nature of the base attached at C1' in these duplexes that have a substantial fraction of residues within alternating purine-pyrimidine sequences. The correlation is primarily associated with changes in the equilibrium distribution of furanose pseudorotational states that may arise in part from the relief of interstrand purine-purine steric clashes.

引用

页码：2430 / 2440

页数：11