COTRANSLATIONAL INTEGRATION OF SOYBEAN (GLYCINE-MAX) OIL BODY MEMBRANE-PROTEIN OLEOSIN INTO MICROSOMAL-MEMBRANES

被引:53
作者
LOER, DS [1 ]
HERMAN, EM [1 ]
机构
[1] USDA ARS,BELTSVILLE AGR RES CTR,PLANT MOLEC BIOL LAB,BELTSVILLE,MD 20705
关键词
D O I
10.1104/pp.101.3.993
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Storage triglycerides in oil seeds are sequestered in discrete organelles termed oil bodies. They are bounded by a monolayer of phospholipids in which a few distinct proteins (oleosins) are embedded. Synthesis of soybean (Glycine max) 24-kD oleosin was analyzed by in vitro transcription and translation in reticulocyte lysate in the presence of canine microsomes. Our results show that 24-kD oleosin is cotranslationally integrated into microsomal membranes. We demonstrate that oleosin is integrated into a bilayer membrane in preference to the oil body monolayer membrane, indicating that oleosin is synthesized on the endoplasmic reticulum (ER). A new model of oil body assembly involving a conformational change through initial association with the ER membrane is proposed.
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收藏
页码:993 / 998
页数:6
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