THE THIOL-SPECIFIC ANTIOXIDANT PROTEIN FROM HUMAN BRAIN - GENE CLONING AND ANALYSIS OF CONSERVED CYSTEINE REGIONS

被引：66

作者：

LIM, YS

CHA, MK

KIM, HK

KIM, IH

机构：

[1] PAI CHAI UNIV,DEPT BIOCHEM,TAEJON 302735,SOUTH KOREA

[2] PAI CHAI UNIV,DEPT GENET ENGN,TAEJON 302735,SOUTH KOREA

来源：

GENE | 1994年 / 140卷 / 02期

关键词：

CDNA; SEQUENCING; FUNCTIONAL EXPRESSION; HOMOLOGOUS PROTEIN; ACTIVATED OXYGEN SPECIES;

D O I：

10.1016/0378-1119(94)90558-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The complete cDNA encoding human thiol-specific antioxidant protein (PRP) was isolated from a human brain cDNA library in the lambdaZap expression vector. An open reading frame (ORF) was identified and found to encode a polypeptide of 197 aa with a M(r) of 21 729. The cDNA contained 98 bp of 5'-untranslated sequence (UTR) and 259 bp of 3'-UTR containing a poly(A) signal, AATAAA. Expression of the human PRP cDNA in Escherichia coli yielded a functionally active protein. The observed local sequence homologies between human PRP and other homologous proteins whose functions have not yet been defined give important insight into elucidating the biochemical function of a new protein family which has highly conserved regions containing cysteine.

引用

页码：279 / 284

页数：6

共 28 条

[21]

TARTAGLIA LA, 1990, J BIOL CHEM, V265, P10535

[22]

THELANDER L, 1970, J BIOL CHEM, V245, P6026

[23] CDNA SEQUENCE-ANALYSIS OF A 29-KDA CYSTEINE-RICH SURFACE-ANTIGEN OF PATHOGENIC ENTAMOEBA-HISTOLYTICA [J].