DETERMINATION OF THE QUANTUM EFFICIENCY OF PHOTOSYSTEM-II AND OF NONPHOTOCHEMICAL QUENCHING OF CHLOROPHYLL FLUORESCENCE IN THE FIELD

被引：423

作者：

BILGER, W

SCHREIBER, U

BOCK, M

机构：

[1] Julius-von-Sachs-Institut für Biowissenschaften der Universität Würzburg, Würzburg, D-97082

来源：

OECOLOGIA | 1995年 / 102卷 / 04期

关键词：

PHOTOSYNTHESIS; PS II QUANTUM YIELD; CHLOROPHYLL FLUORESCENCE; FAGUS SYLVATICA;

D O I：

10.1007/BF00341354

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

A newly developed portable chlorophyll fluorometer in combination with a special leaf clip holder was used for assessing photosynthetic activity of attached sun leaves of Fagus sylvatica and Cucurbita pepo under field conditions. During diurnal time courses, fluorescence yield, photosynthetic photon flux density (PPFD) incident on the leaf plane, and leaf temperature were measured and quantum efficiency of photosystem II (PS II), apparent relative electron transport rates, and non-photochemical fluorescence quenching (NPQ) calculated. In both species, quantum efficiency followed closely the incident PPFD and no hysteresis could be observed during the day. Apparent electron transport rate showed light saturation above a PPFD of 700 mu mol m(-2) s(-1) in F. sylvatica, while in C. pepo no saturation was visible up to 1400 mu mol m(-2) s(-1). NPQ was closely correlated to excessive PPFD calculated from the PS II quantum yield. Maximal NPQ observed was 3.3. Although the beech leaf was exposed for a considerable time to PPFD values of 1400-1500 mu mol m(-2) s(-1) and leaf temperatures between 30 and 35 degrees C, no obvious signs for sustained photodamage could be observed. The data demonstrate the potential of chlorophyll fluorescence measurements to analyse photosynthetic performance under field conditions with minimal disturbance of the plant. Potential error sources due to the geometry of the leaf clip holder used are discussed.

引用

页码：425 / 432

页数：8

共 35 条

[21] Krause G.H., Somersalo S., Zumbusch E., Weyers B., Laasch H., On the mechanism of photoinhibition in chloroplasts. Relationship between changes in fluorescence and activity of photosystem. II, J Plant Physiol, 136, pp. 472-479, (1990)
[22] Long S.P., Humphries S., Falkowski P.G., Photoinhibition of photosynthesis in nature, Annu Rev Plant Phys Plant Mol Biol, 45, pp. 633-662, (1994)
[23] Ludlow M., Light stress at high temperature, Photoinhibition, pp. 89-110, (1987)
[24] Markgraf T., Berry J.A., Measurement of photochemical and non-photochemical quenching. Correction for turnover of PS II during steady state photosynthesis, Current research in photosynthesis, vol 4, pp. 279-282, (1990)
[25] Oberhuber W., Dai Z-Y, Edwards G.E., Light dependence of quantum vields of photosystem II and CO2 fixation in C3 and C4 plants, Photosynth Res, 35, pp. 265-274, (1993)
[26] Quick W.P., Horton P., Studies on the induction of chlorophyll fluorescence in barley protoplasts. II. Resolution of fluorescence quenching by redox state and the transthylakoid pH gradient, Proc R Soc Lond B, 220, pp. 374-382, (1984)
[27] Schreiber U., Bilger W., Assessment of stress effects on plant leaves by chlorophyll fluorescence measurements, Plant response to stress—functional analysis in Mediterranean ecosystems, pp. 27-53, (1987)
[28] Schreiber U., Bilger W., Progress in chlorophyll fluorescence research: major developments during the past years in retrospect, Progress in botany, vol 54, pp. 151-173, (1993)
[29] Schreiber U., Schliwa U., Bilger W., Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer, Photosynth Res, 10, pp. 51-62, (1986)
[30] Schreiber U., Bilger W., Neubauer C., Chlorophyll fluorescence as a non-intrusive indicator for rapid assessment of in vivo photosynthesis, Ecophysiology of photosynthesis, pp. 49-70, (1994)

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