BIODEGRADABLE MICROSPHERES .18. THE ADJUVANT EFFECT OF POLYACRYL STARCH MICROPARTICLES WITH CONJUGATED HUMAN SERUM-ALBUMIN

被引：18

作者：

DEGLING, L ^{[1
]}

STJARNKVIST, P ^{[1
]}

机构：

[1] UNIV UPPSALA,DEPT PHARMACEUT,S-75123 UPPSALA,SWEDEN

来源：

VACCINE | 1995年 / 13卷 / 07期

关键词：

POLYACRYL STARCH MICROPARTICLES; ADJUVANT; HSA; IMMUNIZATION; COVALENT COUPLING; DTH-RESPONSE; ANTIBODY RESPONSE;

D O I：

10.1016/0264-410X(94)00031-H

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The adjuvant effect of polyacryl starch microparticles was examined in mice using covalently coupled human serum albumin as a model antigen. Mice were immunized using different administration routes and the humoral and cellular immune responses were followed. The intraperitoneal immunizations gave the strongest humoral immune response, almost as high as with Freund's Complete Adjuvant (FCA), and the response after intravenous and intramuscular immunizations were somewhat lower. The delayed type hypersensitivity response was, after intramuscular immunization, about the same as after intraperitoneal immunizations with FCA. The results shows that polyacryl starch microparticles is a promising candidate for a new adjuvant for human use.

引用

页码：629 / 636

页数：8

共 20 条

[11] Hjerten, “Molecular sieve” chromatography on polyacrylamide gels prepared according to a simplified method, Arch Biochem Biophys, pp. 147-151, (1962)
[12] Edman, Ekman, Sjoholm, Immobilization of proteins in microspheres of biodegradable polyacryldextran, J. Pharm. Sci., 69, pp. 838-842, (1980)
[13] Bethell, Ayers, Hearn, Hancock, Investigation of the activation of various insoluble polysaccarides with 11′-carbonyldiimidazole and of the properities of the activated matrices, Journal of Chromatography A, 219, pp. 361-372, (1981)
[14] Voller, Bidwell, Bartlett, The Enzyme Linked Immunoabsorbent Assay (ELISA), (1979)
[15] Shek, Heath, Immune respone mediated by liposome-associated protein antigens III. Immunogenicity of bovine serum albumin covalently coupled to vesicle surface, Immunology, 50, pp. 101-106, (1983)
[16] Characteristics and practical use of new-generation adjuvants as an acceptable alternative to Freund's complete adjuvant, 44th Forum in Immunology, 143, pp. 475-585, (1992)
[17] Allison, Byars, Immunological adjuvants: desirable properties and side-effects, Molec. Immunol., 28, pp. 279-284, (1991)
[18] Degling, Stjarnkvist, Sjoholm, Interferon-γ in starch microparticles: nitric-oxide generating activity in vitro and antileishmanial effect in mice, Pharm. Res., 10, pp. 783-790, (1993)
[19] Artursson, Edman, Sjoholm, Biodegradable microspheres I: duration of the action of dextranase entrapped in polyacryl starch microparticles in vivo, J. Pharmacol. Exp. Ther., 231, pp. 705-712, (1984)
[20] Allison, Byars, An adjuvant formulation that selectively elicits the formulation of antibodies of protective isotypes and of cell-mediated immunity, J. Immunol. Meth., 95, pp. 157-168, (1986)

← 1 2 →