ASSESSMENT OF METHANOLYSIS FOR THE DETERMINATION OF SUGARS IN PECTINS

被引：42

作者：

QUEMENER, B

THIBAULT, JF

机构：

[1] Laboratoire de Biochimie et Technologie des Glucides, Institut National de la Recherche Agronomique, Centre de Recherches Agro-alimentaires de Nantes, F-44026 Nantes

来源：

CARBOHYDRATE RESEARCH | 1990年 / 206卷 / 02期

关键词：

D O I：

10.1016/0008-6215(90)80067-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the determination of galacturonic acid and the main neutral sugars in pectins involves enzymic hydrolysis followed by methanolysis and h.p.l.c. The usefulness of this method was demonstrated by comparison of the results obtained by (1) methanolysis in methanolic m HCl without enzymic prehydrolysis, (2) methanolysis in methanolic 72% H2SO4 with pretreatment for 3 h with aqueous 72% H2SO4, (3) colorimetric determination of galacturonic acid, and (4) g.l.c. of the alditol acetates of the neutral sugars released by acid hydrolysis under various conditions. © 1990.

引用

页码：277 / 287

页数：11