SOLUBILIZATION OF WALL-BOUND PEROXIDASES BY LIMITED PROTEOLYSIS

被引：9

作者：

MCDOUGALL, GJ

机构：

[1] Scottish Crop Research Institute, Invergowrie

来源：

PHYTOCHEMISTRY | 1993年 / 33卷 / 04期

关键词：

LINUM-USITATISSIMUM; LINACEAE; FLAX; LIGNIN; CELL WALL-BOUND PEROXIDASES;

D O I：

10.1016/0031-9422(93)85271-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cell wall residue, enriched in lignin, which was obtained from non-xylem tissues of flax stems a er extensive digestion with the glycanase mixture, Driselase, had appreciable peroxidase activity which was tightly bound to the wall fragments. A portion of this residual activity was solubilized by limited proteolysis using trypsin. Non-denaturing gel electrophoresis of the trypsin-solubilized extracts revealed the presence of at least one highly mobile anionic peroxidase isozyme.

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收藏

页码：765 / 767

页数：3

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