DETERMINATION OF THE NEUROLATHYROGEN BETA-N-OXALYL-L-ALPHA,BETA-DIAMINOPROPIONIC ACID USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION

被引:28
作者
GEDA, A
BRIGGS, CJ
VENKATARAM, S
机构
[1] UNIV MANITOBA,FAC PHARM,WINNIPEG R3T 2N2,MANITOBA,CANADA
[2] INDIRA GANDHI AGR UNIV,DEPT PLANT BREEDING & GENET,RAIPUR 492012,MADHYA PRADESH,INDIA
来源
JOURNAL OF CHROMATOGRAPHY | 1993年 / 635卷 / 02期
关键词
D O I
10.1016/0021-9673(93)80378-L
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This paper describes a sensitive spectrofluorometric HPLC method suitable for determining picogram levels of beta-N-oxalyl-L-alpha,beta-diaminopropionic acid (ODAP), a neurotoxin. Aqueous extracts of powdered Lathyrus sativus seeds were treated with 9-fluorenylmethyl chloroformate (FMOC) and the ODAP-FMOC derivative analyzed by reversed-phase chromatography using a mu Bondapak C18 column. The excitation and emission wavelengths were 254 and 315 nm, respectively. The mobile phase was sodium acetate buffer (0.05 M, pH 6.35)-acetonitrile (72:28, v/v) at a flow-rate of 1 ml/min. This method represents a major advance over the standard spectrophotometric assays used currently.
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页码:338 / 341
页数:4
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