DETERMINATION OF THE NEUROLATHYROGEN BETA-N-OXALYL-L-ALPHA,BETA-DIAMINOPROPIONIC ACID USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION

被引：28

作者：

GEDA, A

BRIGGS, CJ

VENKATARAM, S

机构：

[1] UNIV MANITOBA,FAC PHARM,WINNIPEG R3T 2N2,MANITOBA,CANADA

[2] INDIRA GANDHI AGR UNIV,DEPT PLANT BREEDING & GENET,RAIPUR 492012,MADHYA PRADESH,INDIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 635卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)80378-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes a sensitive spectrofluorometric HPLC method suitable for determining picogram levels of beta-N-oxalyl-L-alpha,beta-diaminopropionic acid (ODAP), a neurotoxin. Aqueous extracts of powdered Lathyrus sativus seeds were treated with 9-fluorenylmethyl chloroformate (FMOC) and the ODAP-FMOC derivative analyzed by reversed-phase chromatography using a mu Bondapak C18 column. The excitation and emission wavelengths were 254 and 315 nm, respectively. The mobile phase was sodium acetate buffer (0.05 M, pH 6.35)-acetonitrile (72:28, v/v) at a flow-rate of 1 ml/min. This method represents a major advance over the standard spectrophotometric assays used currently.

引用

页码：338 / 341

页数：4