EFFECT OF VASOACTIVE INTESTINAL POLYPEPTIDE ON PROLACTIN-RELEASE INVITRO

被引：97

作者：

SHAAR, CJ

CLEMENS, JA

DININGER, NB

机构：

[1] The Lilly Research Laboratories, Eli Lilly and Company, Indianapolis

来源：

LIFE SCIENCES | 1979年 / 25卷 / 24-2期

关键词：

D O I：

10.1016/0024-3205(79)90199-1

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Vasoactive intestinal polypeptide (VIP) at concentrations of 1 × 10-8M to 1 × 10-5M caused release of prolactin from anterior pituitary tissue in vitro under specific experimental conditions. VIP stimulated prolactin release when anterior pituitary tissue was incubated in medium 199 in glass culture tubes containing the peptide-antibiotic bacitracin or bovine serum albumin. VIP also caused significant release of prolactin when polystyrene instead of glass culture tubes were used as incubation vessels. The results indicate that VIP can cause prolactin release by a direct action on anterior pituitary lactotrophes. Addition of bacitracin or bovine serum albumin to the culture medium must prevent VIP from binding to glass. Use of polystyrene culture tubes eliminates the need for an extraneous protein source to prevent VIP glass binding. © 1979.

引用

页码：2071 / 2074

页数：4

共 13 条

[1] BRYANT MG, 1976, LANCET, V1, P991
[2] VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) - VESICULAR LOCALIZATION AND POTASSIUM EVOKED RELEASE FROM RAT HYPOTHALAMUS
EMSON, PC
FAHRENKRUG, J
SCHAFFALITZKYDEMUCKADELL, OB
JESSELL, TM
IVERSEN, LL
[J]. BRAIN RESEARCH, 1978, 143 (01) : 174 - 178
[3] FAHRENKRUG J, 1977, J LAB CLIN MED, V89, P1379
[4] GIACHETTI A, 1976, LANCET, V2, P741
[5] PROLACTIN-RELEASE BY VASOACTIVE INTESTINAL POLYPEPTIDE IN RATS
KATO, Y
IWASAKI, Y
IWASAKI, J
ABE, H
YANAIHARA, N
IMURA, H
[J]. ENDOCRINOLOGY, 1978, 103 (02) : 554 - 558
[6] IMMUNOHISTOCHEMICAL LOCALIZATION OF A VASODILATORY POLYPEPTIDE (VIP) IN CEREBROVASCULAR NERVES
LARSSON, LI
EDVINSSON, L
FAHRENKRUG, J
HAKANSON, R
OWMAN, C
SCHAFFALITZKYDEMUCKADELL, O
SUNDLER, F
[J]. BRAIN RESEARCH, 1976, 113 (02) : 400 - 404
[7] LOCALIZATION OF VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) TO CENTRAL AND PERIPHERAL NEURONS
LARSSON, LI
FAHRENKRUG, J
MUCKADELL, OSD
SUNDLER, F
HAKANSON, R
REHFELD, JF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (09) : 3197 - 3200
[8] STRUCTURE OF PORCINE VASOACTIVE INTESTINAL OCTACOSAPEPTIDE - AMINO-ACID SEQUENCE - USE OF KALLIKREIN IN ITS DETERMINATION
MUTT, V
SAID, SI
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 42 (02): : 581 - 589
[9] STIMULATION OF PROLACTIN-RELEASE BY VASOACTIVE INTESTINAL PEPTIDE (VIP)
RUBERG, M
ROTSZTEJN, WH
ARANCIBIA, S
BESSON, J
ENJALBERT, A
[J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1978, 51 (03) : 319 - 320
[10] VASOACTIVE INTESTINAL POLYPEPTIDE - RELEASE INTO HYPOPHYSEAL PORTAL BLOOD
SAID, SI
PORTER, JC
[J]. LIFE SCIENCES, 1979, 24 (03) : 227 - 230

← 1 2 →