DETERMINATION OF LOW PLASMA TIMOLOL CONCENTRATIONS FOLLOWING TOPICAL APPLICATION OF TIMOLOL EYE DROPS IN HUMANS BY HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引：13

作者：

HE, HB ^{[1
]}

EDEKI, TI ^{[1
]}

WOOD, AJJ ^{[1
]}

机构：

[1] VANDERBILT UNIV,SCH MED,DEPT PHARMACOL,NASHVILLE,TN 37232

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 661卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00357-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive high-performance liquid chromatographic assay was developed for determination of timolol in human plasma following administration of two drops of a 5% timolol ophthalmic solution. A 4% butyl alcohol-hexane extract of an alkalized sample of plasma was chromatographed on a reversed-phase column and the components in the column effluent were monitored by coulometric detection. The extraction efficiency of timolol was 69.02 +/- 4.16% (mean +/- S.D.) and its detection limit was 107.2 pg/ml. The effect of mobile phase pH, buffer concentration and the working potential of the detector on column performance and the electrochemical response are described.

引用

页码：351 / 356

页数：6

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