CDNA FROM HUMAN OCULAR CILIARY EPITHELIUM HOMOLOGOUS TO BETA-IG-H3 IS PREFERENTIALLY EXPRESSED AS AN EXTRACELLULAR PROTEIN IN THE CORNEAL EPITHELIUM

被引:147
作者
ESCRIBANO, J
HERNANDO, N
GHOSH, S
CRABB, J
COCAPRADOS, M
机构
[1] YALE UNIV,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,NEW HAVEN,CT 06510
[2] W ALTON JONES CELL SCI CTR,LAKE PLACID,NY 12946
关键词
D O I
10.1002/jcp.1041600314
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The non-pigmented ciliary epithelium is largely responsible for the formation of aqueous humor in the mammalian eye. To provide a basis for studies at the molecular level, a directional expression cDNA library was constructed in Uni-ZAP XR vector from poly A(+) RNA of the human non-pigmented ciliary epithelial derived ODM-2 cell line. Fifty-three cDNA clones were isolated from the library and characterized by partial sequence analysis. Approximately 49% of the clones exhibited homology with known genes in the GenBank/EMBL databases. The putative identification of these clones may reflect the transcriptional activity of the ODM-2 cells in culture. One of the identified clones, ODM-42-I, was found to be specific and highly expressed in the corneal epithelium. This clone had an exact match with a recently discovered human gene, beta ig-h3 (Skonier et al., 1992, DNA Cell Biol., 11:511-522), which codes a surface recognition protein, inducible by transforming growth factor beta (TGF-beta), and containing a putative binding site (RDG) for integrins. The ODM-42-I cDNA clone displays a distinctive pattern of expression found in the human eye, expressed almost exclusively in the cornea. Further studies, using sera from a synthetic peptide to the carboxy-terminal region of ODM-42-I, reveal that the protein is heterogeneous in charge and is preferentially expressed on the extracellular surface of corneal epithelial cells, and might share immunologic properties with integrins beta 1 (C) 1994 Wiley-Liss, Inc.
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页码:511 / 521
页数:11
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