CLONING AND POLYMERASE CHAIN-REACTION SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS OF ANONYMOUS ALU REPEATS ON CHROMOSOME-11

被引：7

作者：

IIZUKA, M

MASHIYAMA, S

OSHIMURA, M

SEKIYA, T

HAYASHI, K

机构：

[1] NATL CANC CTR,RES INST,DIV ONCOGENE,1-1 TSUKIJI 5-CHOME,CHUO KU,TOKYO 104,JAPAN

[2] TOTTORI UNIV,SCH MED,DEPT LIFE SCI,YONAGO,TOTTORI 683,JAPAN

来源：

GENOMICS | 1992年 / 12卷 / 01期

关键词：

D O I：

10.1016/0888-7543(92)90416-P

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have shown that many of the Alu repeats found in the GenBank database are polymorphic and that this polymorphism can be detected by a simple technique, single-strand conformation polymorphism (SSCP) analysis, after polymerase chain reaction (PCR) amplification of each repeat from DNA of individuals. Here, we describe a method for collecting many anonymous Alu repeats and their flanks in a chromosome-specific phage library and cloning them into plasmids. The flanking single-copy sequences of each repeat in the plasmid were then determined, and 20mer to 30mer segments of these sequences were used as primers for the PCR-SSCP analysis. Many new polymorphic DNA markers on chromosome 11 were obtained with this method. These markers can also serve as sequence-tagged sites for physical mapping of the genome. © 1992.

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页码：139 / 146

页数：8

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