CHARACTERISTICS OF DNA AND MULTIPLE RPOD HOMOLOGS OF MICROCYSTIS (SYNECHOCYSTIS) STRAINS

被引：31

作者：

SAKAMOTO, T

SHIRAI, M

ASAYAMA, M

AIDA, T

SATO, A

TANAKA, K

TAKAHASHI, H

NAKANO, M

机构：

[1] IBARAKI UNIV, DIV BIOTECHNOL, AMI, IBARAKI 3003, JAPAN

[2] UNIV TOKYO, INST APPL MICROBIOL, BUNKYO KU, TOKYO 113, JAPAN

[3] JICHI MED SCH, DEPT MICROBIOL, MINAMI KAWACHI, TOCHIGI 32904, JAPAN

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1993年 / 43卷 / 04期

关键词：

D O I：

10.1099/00207713-43-4-844

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The base compositions of DNAs from nine Microcystis strains, as determined by high-performance liquid chromatography, were 41 to 42 mol% G+C. Chromosomal DNAs derived from these strains were found to be extremely resistant to many restriction endonucleases, and a restriction analysis revealed the presence of a dam-like methylase or both dam- and dcm-like methylases in all of the strains examined. Genomic Southern hybridization in which a synthetic oligonucleotide probe (rpoD probe) was used showed that members of the genus Microcystis might have multiple rpoD homologs, and the hybridization signal patterns observed with the DNAs of Microcystis aeruginosa strains were different from each other.

引用

页码：844 / 847

页数：4

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[11] RESISTANCE OF DNA FROM FILAMENTOUS AND UNICELLULAR CYANOBACTERIA TO RESTRICTION ENDONUCLEASE CLEAVAGE [J].