A FACILE METHOD TO DETERMINE THE CO2/O2 SPECIFICITY FACTOR FOR RIBULOSE-BISPHOSPHATE CARBOXYLASE OXYGENASE

被引：17

作者：

LEE, GJ ^{[1
]}

KOSTOV, RV ^{[1
]}

MCFADDEN, BA ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,DEPT BIOCHEM & BIOPHYS,PULLMAN,WA 99164

来源：

PHOTOSYNTHESIS RESEARCH | 1993年 / 37卷 / 01期

关键词：

RIBULOSE 1,5-BISPHOSPHATE ASSAY; RUBISCO; SPECIFICITY FACTOR; TAU;

D O I：

10.1007/BF02185441

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A rapid method to determine the CO2/O2 specificity factor of ribulose 1,5-bisphosphate carboxylase/oxygenase is presented. The assay measures the amount of CO2 and O2 fixation at varying CO2/O2 ratios to determine the relative rates of each reaction. CO2 fixation is measured by the incorporation of the moles of (CO2)-C-14 into 3-phosphoglycerate, while O2 fixation is determined by subtraction of the moles of CO2 fixed from the moles of RuBP consumed in each reaction. By analyzing the inorganic phosphate specifically hydrolyzed from RuBP under alkaline conditions, the amount of RuBP present before and after catalysis by rubisco can be determined.

引用

页码：81 / 86

页数：6

共 18 条

[11] VARIATION IN THE SPECIFICITY FACTOR OF C-3 HIGHER-PLANT RUBISCO DETERMINED BY THE TOTAL CONSUMPTION OF RIBULOSE-P-2 [J].

PARRY, MAJ ;

KEYS, AJ ;

GUTTERIDGE, S .

JOURNAL OF EXPERIMENTAL BOTANY, 1989, 40 (212) :317-320

[12]

PARRY MAJ, 1992, PLANTA, V187, P109, DOI 10.1007/BF00201631

[13] STOICHIOMETRY IN THE ASSAY OF RIBULOSE BISPHOSPHATE OXYGENASE AND CARBOXYLASE [J].

PUROHIT, K ;

MCFADDEN, BA ;

SALUJA, A .

ANALYTICAL BIOCHEMISTRY, 1982, 124 (01) :158-166

[14] A HYBRID RIBULOSEBISPHOSPHATE CARBOXYLASE OXYGENASE ENZYME EXHIBITING A SUBSTANTIAL INCREASE IN SUBSTRATE-SPECIFICITY FACTOR [J].

READ, BA ;

TABITA, FR .

BIOCHEMISTRY, 1992, 31 (24) :5553-5560

[15] RIBULOSE BISPHOSPHATE CARBOXYLASE OXYGENASE IN TOLUENE-PERMEABILIZED RHODOSPIRILLUM-RUBRUM [J].

STORRO, I ;

MCFADDEN, BA .

BIOCHEMICAL JOURNAL, 1983, 212 (01) :45-54

[16]

WONG CH, 1982, METHOD ENZYMOL, V89, P108

[17] CORRECT PK VALUES FOR DISSOCIATION-CONSTANT OF CARBONIC-ACID LOWER THE REPORTED KM VALUES OF RIBULOSE BISPHOSPHATE CARBOXYLASE TO HALF - PRESENTATION OF A NOMOGRAPH AND AN EQUATION FOR DETERMINING THE PK VALUES [J].

YOKOTA, A ;

KITAOKA, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 131 (03) :1075-1079

[18] SIMPLE DETERMINATION OF THE CO2/O-2 SPECIFICITY OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE BY THE SPECIFIC RADIOACTIVITY OF [C-14] GLYCERATE 3-PHOSPHATE [J].

ZHU, GH ;

JENSEN, RG ;

HALLICK, RB ;

WILDNER, GF .

PLANT PHYSIOLOGY, 1992, 98 (02) :764-768

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