SITE-SPECIFIC DISULFIDE BRIDGES IN OLIGODEOXYRIBONUCLEOTIDE DUPLEXES CONTAINING 6-MERCAPTOPURINE AND 4-THIOTHYMINE BASES

被引：33

作者：

MILTON, J

CONNOLLY, BA

NIKIFOROV, TT

COSSTICK, R

机构：

[1] DEPT CHEM,ROBERT ROBINSON LABS,POB 147,LIVERPOOL L69 3BX,ENGLAND

[2] UNIV SOUTHAMPTON,INST BIOMOLEC SCI,DEPT BIOCHEM,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND

[3] UNIV NEWCASTLE UPON TYNE,DEPT BIOCHEM & GENET,NEWCASTLE TYNE NE2 4HH,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1993年 / 09期

关键词：

D O I：

10.1039/c39930000779

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A self complementary oligodeoxynucleotide duplex containing 6-thio-2'-deoxyinosine and 4-thio-2'-deoxythymidine undergoes cross-linking to form interstrand disulfide bridges under aerobic conditions; the reaction is reversed by dithiothreitol.

引用

页码：779 / 780

页数：2

共 12 条

[1] DUPLEX OLIGODEOXYRIBONUCLEOTIDES CROSS-LINKED BY MITOMYCIN-C AT A SINGLE SITE - SYNTHESIS, PROPERTIES, AND CROSS-LINK REVERSIBILITY [J].

BOROWYBOROWSKI, H ;

LIPMAN, R ;

CHOWDARY, D ;

TOMASZ, M .

BIOCHEMISTRY, 1990, 29 (12) :2992-2999

[2] SYNTHESIS OF A DINUCLEOSIDE MONOPHOSPHATE ANALOG CONTAINING 6-N-(2-AMINOETHYL)-2'-DEOXYADENOSINE - A NOVEL-APPROACH TO SEQUENCE SPECIFIC CROSS-LINKING IN SYNTHETIC OLIGONUCLEOTIDES [J].

COSSTICK, R ;

DOUGLAS, ME .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1991, (05) :1035-1040

[3] A NOVEL COMBINED CHEMICAL ENZYMATIC-SYNTHESIS OF CROSS-LINKED DNA USING A NUCLEOSIDE TRIPHOSPHATE ANALOG [J].

COWART, M ;

BENKOVIC, SJ .

BIOCHEMISTRY, 1991, 30 (03) :788-796

[4] DISULFIDE CROSS-LINKED OLIGONUCLEOTIDES [J].

FERENTZ, AE ;

VERDINE, GL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (10) :4000-4002

[5] TRAPPING AND ISOLATION OF AN ALTERNATE DNA CONFORMATION [J].

GLICK, GD ;

OSBORNE, SE ;

KNITT, DS ;

MARINO, JP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (13) :5447-5448

[6] INTERACTION OF THE ECORV RESTRICTION ENDONUCLEASE WITH THE DEOXYADENOSINE AND THYMIDINE BASES IN ITS RECOGNITION HEXAMER D(GATATC) [J].

NEWMAN, PC ;

WILLIAMS, DM ;

COSSTICK, R ;

SEELA, F ;

CONNOLLY, BA .

BIOCHEMISTRY, 1990, 29 (42) :9902-9910

[7] THE SYNTHESIS OF OLIGODEOXYNUCLEOTIDES CONTAINING 4-THIOTHYMIDINE RESIDUES [J].

NIKIFOROV, TT ;

CONNOLLY, BA .

TETRAHEDRON LETTERS, 1991, 32 (31) :3851-3854

[8] PREPARATION OF A NOVEL PSORALEN CONTAINING DEOXYADENOSINE BUILDING BLOCK FOR THE FACILE SOLID-PHASE SYNTHESIS OF PSORALEN-MODIFIED OLIGONUCLEOTIDES FOR A SEQUENCE SPECIFIC CROSSLINK TO A GIVEN TARGET SEQUENCE [J].

PIELES, U ;

SPROAT, BS ;

NEUNER, P ;

CRAMER, F .

NUCLEIC ACIDS RESEARCH, 1989, 17 (22) :8967-8978

[9] NUCLEIC-ACID RELATED COMPOUNDS .8. DIRECT CONVERSION OF 2'-DEOXYINOSINE TO 6-CHLOROPURINE 2'-DEOXYRIBOSIDE AND SELECTED 6-SUBSTITUTED DEOXYNUCLEOSIDES AND THEIR EVALUATION AS SUBSTRATES OF ADENOSINE DEAMINASE [J].

ROBINS, MJ ;

BASOM, GL .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1973, 51 (19) :3161-3169

[10] POLYMER SUPPORT OLIGONUCLEOTIDE SYNTHESIS .18. USE OF BETA-CYANOETHYL-N,N-DIALKYLAMINO-/N-MORPHOLINO PHOSPHORAMIDITE OF DEOXYNUCLEOSIDES FOR THE SYNTHESIS OF DNA FRAGMENTS SIMPLIFYING DEPROTECTION AND ISOLATION OF THE FINAL PRODUCT [J].

SINHA, ND ;

BIERNAT, J ;

MCMANUS, J ;

KOSTER, H .

NUCLEIC ACIDS RESEARCH, 1984, 12 (11) :4539-4557

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