PROTEIN ENGINEERING AND THE STUDY OF STRUCTURE-FUNCTION-RELATIONSHIPS IN RECEPTORS

被引：44

作者：

WARD, WHJ ^{[1
]}

TIMMS, D ^{[1
]}

FERSHT, AR ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,CHEM LAB,CAMBRIDGE CB2 1EW,ENGLAND

来源：

TRENDS IN PHARMACOLOGICAL SCIENCES | 1990年 / 11卷 / 07期

关键词：

D O I：

10.1016/0165-6147(90)90009-W

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Protein engineering is a powerful tool for studying relationships between receptor structure and function - providing that it is used and interpretation appropriately. Site-directed mutagenesis, deletion mutagenesis and construction of chimaeric proteins have all been used to characterize receptors. In this review, Walter Ward, David Timms and Alan Fersht describe the application of protein engineering, illustrating important concepts with experimental data. They explain that detailed study of function requires careful dissection of mechanistic steps. Care must also be taken when selecting replacement residues; mutation should not cause delocalized structural reorganization or else the true significance of functional change will remain unclear. © 1990.

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页码：280 / 284

页数：5

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