ISOLATION AND CHARACTERIZATION OF A NOVEL INTERFERON-ALPHA-ENCODING GENE, IFN-ALPHA-11, WITHIN A MURINE IFN CLUSTER

被引：25

作者：

COULOMBEL, C ^{[1
]}

VODJDANI, G ^{[1
]}

DOLY, J ^{[1
]}

机构：

[1] INST RECH SCI CANC,CNRS,UPR 37,GRP LABS,BP 8,F-94802 VILLEJUIF,FRANCE

来源：

GENE | 1991年 / 104卷 / 02期

关键词：

COSMID LIBRARY; GENOMIC ORGANIZATION; RECOMBINANT DNA; EXPRESSION; TRANSCRIPTION; VIRUS INDUCIBILITY;

D O I：

10.1016/0378-1119(91)90249-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two recombinant cosmids containing three complete murine interferon-alpha-encoding genes (Mu IFN-alpha) have been isolated from a mouse cosmid library. The cluster organization of these genes has been determined. A new Mu IFN-alpha gene (Mu IFN-alpha-11) has been isolated and studied with respect to its structure and inducible transcription pattern. The nucleotide and deduced amino acid sequences of the Mu IFN-alpha-11 gene, as compared to the two other IFN-alpha-7 and IFN-alpha-8 genes, show that, albeit highly homologous, these genes are all different. The transient expression of the three genes gave rise to proteins showing antiviral properties which were neutralized with murine anti-IFN-alpha antibodies. The transcription of the Mu IFN-alpha genes was studied in two uninduced or Newcastle-disease-virus-induced murine cell types. Mu IFN-alpha-11, as well as alpha-7 and alpha-8, are not expressed in L929 nor in C243 cells upon viral induction and therefore constitute an interesting model to study Mu IFN-alpha gene repression.

引用

页码：187 / 195

页数：9