ISOTACHOPHORESIS OF PROTEINS IN UNCOATED OPEN-TUBULAR FUSED-SILICA CAPILLARIES WITH A SIMPLE APPROACH FOR COLUMN CONDITIONING

被引:15
作者
GEBAUER, P
THORMANN, W
机构
[1] UNIV BERN, DEPT CLIN PHARMACOL, MURTENSTR 35, CH-3010 BERN, SWITZERLAND
[2] CZECHOSLOVAK ACAD SCI, INST ANALYT CHEM, CS-61142 BRNO, CZECHOSLOVAKIA
来源
JOURNAL OF CHROMATOGRAPHY | 1991年 / 558卷 / 02期
关键词
D O I
10.1016/0021-9673(91)80009-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The isotachophoretic determination of proteins in uncoated open-tubular fused-silica capillaries of 50 and 75-mu-m I.C. with on-column multi-wavelength detection is reported. Small amounts of hydroxypro-pylmethylcellulose added to the leader provide an efficient method of dynamic column conditioning which allows the high-resolution isotachophoretic determination of most proteins to be performed in the presence of an electro-osmotic flow. Different approaches for cationic and anionic analyses are discussed and illustrated with selected examples.
引用
收藏
页码:423 / 429
页数:7
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