ISOTACHOPHORESIS OF PROTEINS IN UNCOATED OPEN-TUBULAR FUSED-SILICA CAPILLARIES WITH A SIMPLE APPROACH FOR COLUMN CONDITIONING

被引：15

作者：

GEBAUER, P

THORMANN, W

机构：

[1] UNIV BERN, DEPT CLIN PHARMACOL, MURTENSTR 35, CH-3010 BERN, SWITZERLAND

[2] CZECHOSLOVAK ACAD SCI, INST ANALYT CHEM, CS-61142 BRNO, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 558卷 / 02期

关键词：

D O I：

10.1016/0021-9673(91)80009-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The isotachophoretic determination of proteins in uncoated open-tubular fused-silica capillaries of 50 and 75-mu-m I.C. with on-column multi-wavelength detection is reported. Small amounts of hydroxypro-pylmethylcellulose added to the leader provide an efficient method of dynamic column conditioning which allows the high-resolution isotachophoretic determination of most proteins to be performed in the presence of an electro-osmotic flow. Different approaches for cationic and anionic analyses are discussed and illustrated with selected examples.

引用

页码：423 / 429

页数：7

共 15 条

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