INTERLEUKIN-3 REGULATES DEVELOPMENT OF THE 5-LIPOXYGENASE/LEUKOTRIENE C-4 SYNTHASE PATHWAY IN MOUSE MAST-CELLS

被引:92
作者
MURAKAMI, M
AUSTEN, KF
BINGHAM, CO
FRIEND, DS
PENROSE, JF
ARM, JP
机构
[1] BRIGHAM & WOMENS HOSP,DEPT RHEUMATOL & IMMUNOL,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115
关键词
D O I
10.1074/jbc.270.39.22653
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To study cytokine regulation of the 5-lipoxygenase (B-LO)/leukotriene (LT) synthase pathway we have developed mouse bone marrow-derived mast cells (BMMC) that minimally express each protein of the pathway by using a novel culture system, lacking interleukin (IL)-3. When mouse bone marrow cells were cultured for 5 weeks with 100 ng/ml c-kit ligand (KL) and 10 units/ml IL-10, a population of >95% mast cells was obtained, These cells generated 8.3 +/- 4.5 ng of LTC(4)/10(6) cells and 8.1 +/- 2.4 ng of prostaglandin (PG) D-2/10(6) cells after IgE-dependent activation, When these BMMC were cultured for 2-5 weeks more with 100 units/ml IL-3 in the continued presence of KL and IL-10, the IgE-dependent generation of LTC(4) and PGD(2) increased to 212 +/- 36 and 25.5 +/- 8.6 ng/10(6) cells, respectively. The dramatic increase in the IgE-dependent generation of LTC(4) in response to IL-3 was accompanied by a concomitant increase in expression of 5-LO and 5-LO-activating protein and preceded the increased expression of cytosolic phospholipase A, and LTC, synthase. The recognition that IL-3 up-regulates the expression of each protein of the 5-LO pathway for the generation of LTC(4) contrasts with our recent finding that KL up-regulates the expression of cytosolic phospholipase A(2), prostaglandin endoperoxide synthase-1, and hematopoietic PGD(2) synthase and increases the IgE-dependent generation of PGD(2) in BMMC developed from bone marrow with IL-3. Thus, developmentally segregated regulation of the prostanoid and cysteinyl leukotriene pathways in lineage-related com mitted mast cell progenitors reveals the pleiotropism of this effector cell of allergic inflammation, a cytokine/growth factor basis for preferential expression of pathways of eicosanoid biosynthesis, and the particular role of IL-3 in regulating the expression of the proteins of the 5-LO/LTC(4) synthase pathway.
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页码:22653 / 22656
页数:4
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