EXPRESSION OF HUMAN EPIDERMAL GROWTH-FACTOR BY ESCHERICHIA-COLI IN CONTINUOUS CULTURE

被引：8

作者：

KIM, E

FU, J

WILSON, DB

SHULER, ML

机构：

[1] CORNELL UNIV,SCH CHEM ENGN,ITHACA,NY 14853

[2] CORNELL UNIV,BIOCHEM MOLEC & CELL BIOL SECT,ITHACA,NY 14853

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 05期

关键词：

D O I：

10.1007/BF01021244

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Expression of human epidermal growth factor could not be sustained in single-stage chemostats for even one residence time. Selection for low producing variants was observed in which alterations to both the host and plasmid were detected. Continuous expression of hEGF was demonstrated in two-stage chemostats by separating growth and product formation.

引用

收藏

页码：339 / 344

页数：6

相关论文

共 13 条

[1] MECHANISM OF ACTION OF THE LEXA GENE-PRODUCT [J].

BRENT, R ;

PTASHNE, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (07) :4204-4208

[2] ENHANCEMENT OF WOUND-HEALING BY TOPICAL TREATMENT WITH EPIDERMAL GROWTH-FACTOR [J].

BROWN, GL ;

NANNEY, LB ;

GRIFFEN, J ;

CRAMER, AB ;

YANCEY, JM ;

CURTSINGER, LJ ;

HOLTZIN, L ;

SCHULTZ, GS ;

JURKIEWICZ, MJ ;

LYNCH, JB .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (02) :76-79

[3] EFFECTS OF TEMPERATURE ON ESCHERICHIA-COLI OVERPRODUCING BETA-LACTAMASE OR HUMAN EPIDERMAL GROWTH-FACTOR [J].

CHALMERS, JJ ;

KIM, E ;

TELFORD, JN ;

WONG, EY ;

TACON, WC ;

SHULER, ML ;

WILSON, DB .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (01) :104-111

[4] CLOSE SIMILARITY OF EPIDERMAL GROWTH-FACTOR RECEPTOR AND V-ERB-B ONCOGENE PROTEIN SEQUENCES [J].

DOWNWARD, J ;

YARDEN, Y ;

MAYES, E ;

SCRACE, G ;

TOTTY, N ;

STOCKWELL, P ;

ULLRICH, A ;

SCHLESSINGER, J ;

WATERFIELD, MD .

NATURE, 1984, 307 (5951) :521-527

[5]

ENGLER DA, 1988, J BIOL CHEM, V263, P12384

[6]

FU J, 1992, UNPUB BIOTECHNOL PRO

[7] THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA-COLI [J].

HIROTA, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1960, 46 (01) :57-64

[8] EXPRESSION AND SECRETION OF HUMAN EPIDERMAL GROWTH-FACTOR IN ESCHERICHIA-COLI [J].

ITO, A ;

KATOH, T ;

GOMI, H ;

KISHIMOTO, F ;

AGUI, H ;

OGINO, S ;

YODA, K ;

YAMASAKI, M ;

TAMURA, G .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (06) :1381-1388

[9] MASS-PRODUCTION OF HUMAN EPIDERMAL GROWTH-FACTOR USING FED-BATCH CULTURES OF RECOMBINANT ESCHERICHIA-COLI [J].

SHIMIZU, N ;

FUKUZONO, S ;

HARADA, Y ;

FUJIMORI, K ;

GOTOH, K ;

YAMAZAKI, Y .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (01) :37-42

[10] REPLACEMENT OF A PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE BY A NICOTINAMIDE ADENINE DINUCLEOTIDE-LINKED DEHYDROGENASE FOR UTILIZATION OF MANNITOL [J].

TANAKA, S ;

LERNER, SA ;

LIN, ECC .

JOURNAL OF BACTERIOLOGY, 1967, 93 (02) :642-&