EXPRESSION OF HUMAN EPIDERMAL GROWTH-FACTOR BY ESCHERICHIA-COLI IN CONTINUOUS CULTURE

被引：8

作者：

KIM, E

FU, J

WILSON, DB

SHULER, ML

机构：

[1] CORNELL UNIV,SCH CHEM ENGN,ITHACA,NY 14853

[2] CORNELL UNIV,BIOCHEM MOLEC & CELL BIOL SECT,ITHACA,NY 14853

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 05期

关键词：

D O I：

10.1007/BF01021244

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Expression of human epidermal growth factor could not be sustained in single-stage chemostats for even one residence time. Selection for low producing variants was observed in which alterations to both the host and plasmid were detected. Continuous expression of hEGF was demonstrated in two-stage chemostats by separating growth and product formation.

引用

页码：339 / 344

页数：6