A SIMPLIFIED AND RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR PILOCARPINE HYDROCHLORIDE

被引:7
作者
DESAI, SD [1 ]
BLANCHARD, J [1 ]
机构
[1] UNIV ARIZONA,COLL PHARM,DEPT PHARMACEUT SCI,TUCSON,AZ 85721
关键词
D O I
10.1093/chromsci/30.4.149
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A high-performance liquid chromatographic (HPLC) procedure for determination of pilocarpine hydrochloride in aqueous- based pharmaceutical formulations has been developed. Previously reported HPLC assays for pilocarpine hydrochloride involve the use of very low pH (pH 2.5) and a high buffer concentration (5% w/v), both of which can shorten HPLC column life. The present assay for pilocarpine hydrochloride utilizes a reversed-phase ODS column and ultraviolet detection at 220 nm. Clonidine hydrochloride is used as an internal standard in the procedure. The mobile phase consists of pH 3.5, 0.5% w/v phosphate buffer-methanol (70:30) used at a flow rate of 1.25 mL/min. The calibration curves are linear (r = 0.995) over the 4 to 100 µg/mL range. The retention times for pilocarpine hydrochloride and the internal standard are 2.51 and 4.25 min, respectively. The run time per sample is 6 min. The minimum detectable concentration of pilocarpine hydrochloride is 100 ng/mL. The assay is simple, rapid, and reproducible. Details of the specificity, accuracy, reproducibility, and linearity of the HPLC assay are described. © 1992 Oxford University Press.
引用
收藏
页码:149 / 152
页数:4
相关论文
共 26 条
[21]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR PILOCARPINE IN AQUEOUS-HUMOR [J].
RENDI, MA ;
ELLIS, PP ;
GAL, J .
JOURNAL OF CHROMATOGRAPHY, 1984, 336 (02) :337-344
[22]   QUANTITATIVE ANALYSIS OF MICROGRAM QUANTITIES OF PILOCARPINE IN AQUEOUS SOLUTION [J].
REPTA, A ;
HIGUCHI, T .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1971, 60 (10) :1465-&
[23]   SIMULTANEOUS DETERMINATION OF PILOCARPINE AND ISOPILOCARPINE IN PHARMACEUTICAL PREPARATIONS BY LIQUID-CHROMATOGRAPHY [J].
URBANYI, T ;
PIEDMONT, A ;
WILLIS, E ;
MANNING, G .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1976, 65 (02) :257-260
[24]   HIGH-PERFORMANCE CHROMATOGRAPHIC DETERMINATION OF PILOCARPINE AND PILOCARPIC ACID IN OCULAR-TISSUES [J].
WOOD, RW ;
ROBINSON, JR .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1984, 20 (03) :285-293
[25]  
YOSHIOKA S, 1986, CHEM PHARM BULL, V34, P4280
[26]  
1980, USP XX NF XV UNITED, P627