HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSES OF COMPOUNDS RELATED TO NUCLEIC-ACIDS BY 3-(1-NAPHTHOYLAMINO)-PROPYL-MODIFIED AND 3-(1-ANTHROYLAMINO)-PROPYL-MODIFIED SILICA-GEL PACKING MATERIALS

The development of column packing materials was tried as a contribution to studies related to the further modification of 3-aminopropyl-modified silica gel. Consequently, we prepared two types of gel, 3-(1-naphthoylamino)- and 3-(1-anthroylamino)-propyl-modified silica gels (NAPS and AAPS). Since NAPS and AAPS, each having an aromatic ring, were expected to behave in a separation mode by pi-pi or hydrophobic interaction, their application to the analyses of the compounds related to nucleic acids was carried out. Adenosine nucleotides were consequently separated by the gels in a single analysis by a single buffer elution with UV detection. NAPS could be also successfully used for the measurement of ATPase activity in fish meat (Sillago Japonica). The mobile phase consistently used in the analysis was an aqueous phosphate buffer.