CLONING, SEQUENCE, AND PHOTOREGULATION OF AL-1, A CAROTENOID BIOSYNTHETIC GENE OF NEUROSPORA-CRASSA

被引:149
作者
SCHMIDHAUSER, TJ
LAUTER, FR
RUSSO, VEA
YANOFSKY, C
机构
[1] STANFORD UNIV,DEPT BIOL SCI,STANFORD,CA 94305
[2] MAX PLANCK INST MOLEC GENET,TRAUTNER ABT,W-1000 BERLIN 33,GERMANY
关键词
D O I
10.1128/MCB.10.10.5064
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Carotenid biosynthesis is regulated by blue light during growth of Neurospora crassa mycelia. We have cloned the al-1 gene of N. crassa encoding the carotenid-biosynthetic enzyme phytoene dehydrogenase and present an analysis of its structure and regulation. The gene encodes a 595-residue polypeptide that shows homology to two procaryotic carotenoid dehydrogenases. RNA measurements showed that the level of al-1 mRNA increased over 70-fold in photoinduced mycelia. Transcription run-on studies indicated that the al-1 gene was regulated at the level of initiation of transcription in response to photoinduction. The photoinduced increase of al-1 mRNA levels was not observed in two Neurospora mutants defective in all physiological photoresponses. Analysis of a cosmid containing al-1 and of a translocation strain with a breakpoint within al-1 indicated that al-1 transcription proceeds towards the centromere of linkage group I of N. crassa.
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页码:5064 / 5070
页数:7
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