CAPILLARY ELECTROPHORESIS OF HEMOGLOBINS AND GLOBIN CHAINS

被引：75

作者：

ZHU, M ^{[1
]}

RODRIGUEZ, R ^{[1
]}

WEHR, T ^{[1
]}

SIEBERT, C ^{[1
]}

机构：

[1] BIO RAD LABS INC,LIFE SCI GRP,DIV ANALYT SYST,2000 ALFRED NOBEL DR,HERCULES,CA 94547

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 608卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)87128-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary isoelectric focusing (cIEF) and free zone capillary electrophoresis were evaluated for separation of native hemoglobins and globin chains. High-resolution separations of adult human hemoglobin A, fetal human hemoglobin F, and hemoglobin variants S and C were obtained using cIEF with cathodic mobilization. Absorbance detection in the UV and visible regions were compared, and on-line fast UV or visible-wavelength scanning detection was used to obtain spectral information on separated components. Globin chain analysis was performed on the same hemoglobin species by free zone capillary electrophoresis following precipitation of the protein with acidic acetone. Free zone separations were carried out at low pH in the presence of 7 M urea.

引用

页码：225 / 237

页数：13