GUIDING THE SELECTION OF HUMAN-ANTIBODIES FROM PHAGE DISPLAY REPERTOIRES TO A SINGLE EPITOPE OF AN ANTIGEN

被引:175
作者
JESPERS, LS
ROBERTS, A
MAHLER, SM
WINTER, G
HOOGENBOOM, HR
机构
[1] CAMBRIDGE ANTIBODY TECHNOL LTD,MELBOURNE SG8 6E1,CAMBS,ENGLAND
[2] MRC,CTR PROT ENGN,CAMBRIDGE CB2 2QH,ENGLAND
来源
BIO-TECHNOLOGY | 1994年 / 12卷 / 09期
关键词
D O I
10.1038/nbt0994-899
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have developed a strategy for guiding the selection of human antibody fragments from phage display repertoires to a single epitope of an antigen, using rodent monoclonal antibodies as a template. Thus the heavy chain of a rodent antibody (MAb32) directed against human tumor necrosis factor alpha (TNF alpha) was cloned and paired as a template chain with a repertoire of human light chains for display as Fab fragments on filamentous phage. The phage were selected by binding to the antigen. The selected human light chains. were in turn paired with a repertoire of human heavy chains displayed on phage, and the phage selected again. The isolated phage displaying human antibody fragments binding to TNF alpha also bound to a peptide comprising the N-terminal region of TNF alpha as with MAb32. One of the human Fab fragments was recloned for expression as a glycosylated human antibody in mammalian cells: Binding to TNF alpha could be competed with MAb32 or with anti-serum to the peptide, indicating the same epitope. The human antibody was found to have a binding affinity (K-d=15 nM) similar to MAb32 (K-d=26 nM). The process contrasts with existing means of ''humanizing'' rodent monoclonal antibodies in that the antibodies derived are completely human.
引用
收藏
页码:899 / 903
页数:5
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