AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN PHOSPHOLIPASE C-MEDIATED INOSITOL LIPID SIGNALING

被引：193

作者：

THOMAS, GMH ^{[1
]}

CUNNINGHAM, E ^{[1
]}

FENSOME, A ^{[1
]}

BALL, A ^{[1
]}

TOTTY, NF ^{[1
]}

TRUONG, O ^{[1
]}

HSUAN, JJ ^{[1
]}

COCKCROFT, S ^{[1
]}

机构：

[1] UCL, SCH MED, LUDWIG INST CANC RES, LONDON W1P 8BT, ENGLAND

来源：

CELL | 1993年 / 74卷 / 05期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0092-8674(93)90471-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transmembrane signaling by the phospholipase C-beta (PLC-beta) pathway is known to require at least three components: the receptor, the G protein, and the PLC. Recent studies have indicated that if the cytosol is allowed to leak out of HL60 cells, then G protein-stimulated PLC activity is greatly diminished, indicating an essential role for a cytosolic component(s). We now report the complete purification of one component based on its ability to reconstitute GTPgammaS-mediated PLC activity and identify it as the phosphatidylinositol transfer protein (PI-TP). Based on the in vitro effects of PI-TP, we surmise that it is involved in transporting PI from intracellular compartments for conversion to PI bisphosphate (PIP2) prior to hydrolysis by PLC-beta2/PLC-beta3, the endogenous PLC isoforms present in these cells.

引用

页码：919 / 928

页数：10

共 43 条

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