CLONING AND CHARACTERIZATION OF AN ALPHA-TUBULIN-ENCODING GENE FROM RAT-DERIVED PNEUMOCYSTIS-CARINII

被引：32

作者：

ZHANG, JX ^{[1
]}

STRINGER, JR ^{[1
]}

机构：

[1] UNIV CINCINNATI,COLL MED,DEPT MOLEC GENET BIOCHEM & MICROBIOL,CINCINNATI,OH 45267

来源：

GENE | 1993年 / 123卷 / 01期

关键词：

RECOMBINANT DNA; AIDS; FUNGUS; PATHOGEN; DNA;

D O I：

10.1016/0378-1119(93)90553-F

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene and cDNA (TUB1) encoding an alpha-tubulin (alpha-Tub) from Pneumocystis carinii carried in rats were isolated and sequenced. The gene produced a 1.4-kb transcript that contained an open reading frame of 1347 nucleotides coding for a protein of 50 326 Da. The nt sequence of TUBI of P. carinii was 65-71% identical to other TUB1 genes, including those of yeast and human. The deduced amino acid sequence of P. carinii alpha-Tub showed 75-84% identity to other alpha-Tubs. Eight small introns resided within TUB1. P. carinii TUB1 was mapped to a 425-kb chromosome. The cloned gene appears to be the only alpha-Tub-encoding gene in the P. carinii genome.

引用

页码：137 / 141

页数：5

共 14 条

[1]

AUSUBEL FM, 1989, CURRENT PROTOCOLS MO

[2] ELECTROPHORETIC SEPARATIONS OF LARGE DNA-MOLECULES BY PERIODIC INVERSION OF THE ELECTRIC-FIELD [J].

CARLE, GF ;

FRANK, M ;

OLSON, MV .

SCIENCE, 1986, 232 (4746) :65-68

[3] 2 ALPHA-TUBULIN GENES OF ASPERGILLUS-NIDULANS ENCODE DIVERGENT PROTEINS [J].

DOSHI, P ;

BOSSIE, CA ;

DOONAN, JH ;

MAY, GS ;

MORRIS, NR .

MOLECULAR & GENERAL GENETICS, 1991, 225 (01) :129-141

[4] ISOLATION AND EXPRESSION OF THE PNEUMOCYSTIS-CARINII DIHYDROFOLATE-REDUCTASE GENE [J].

EDMAN, JC ;

EDMAN, U ;

CAO, M ;

LUNDGREN, B ;

KOVACS, JA ;

SANTI, DV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8625-8629

[5] ISOLATION AND EXPRESSION OF THE PNEUMOCYSTIS-CARINII THYMIDYLATE SYNTHASE GENE [J].

EDMAN, U ;

EDMAN, JC ;

LUNDGREN, B ;

SANTI, DV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) :6503-6507

[6] THE SEQUENCE 5'-AAUAAA-3' FORMS PART OF THE RECOGNITION SITE FOR POLYADENYLATION OF LATE SV40 MESSENGER-RNAS [J].

FITZGERALD, M ;

SHENK, T .

CELL, 1981, 24 (01) :251-260

[7] PNEUMOCYSTIS-CARINII KARYOTYPES [J].

HONG, ST ;

STEELE, PE ;

CUSHION, MT ;

WALZER, PD ;

STRINGER, SL ;

STRINGER, JR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (08) :1785-1795

[8]

KIMMEL BE, 1985, GENE, V35, P237, DOI 10.1016/0378-1119(85)90002-2

[9] THE SEQUENCES OF AN EXPRESSED RAT ALPHA-TUBULIN GENE AND A PSEUDOGENE WITH AN INSERTED REPETITIVE ELEMENT [J].

LEMISCHKA, I ;

SHARP, PA .

NATURE, 1982, 300 (5890) :330-335

[10] A TANDEM OF ALPHA-TUBULIN GENES PREFERENTIALLY EXPRESSED IN RADICULAR TISSUES FROM ZEA-MAYS [J].

MONTOLIU, L ;

RIGAU, J ;

PUIGDOMENECH, P .

PLANT MOLECULAR BIOLOGY, 1990, 14 (01) :1-15

← 1 2 →