CLONING AND CHARACTERIZATION OF AN ALPHA-TUBULIN-ENCODING GENE FROM RAT-DERIVED PNEUMOCYSTIS-CARINII

被引：32

作者：

ZHANG, JX ^{[1
]}

STRINGER, JR ^{[1
]}

机构：

[1] UNIV CINCINNATI,COLL MED,DEPT MOLEC GENET BIOCHEM & MICROBIOL,CINCINNATI,OH 45267

来源：

GENE | 1993年 / 123卷 / 01期

关键词：

RECOMBINANT DNA; AIDS; FUNGUS; PATHOGEN; DNA;

D O I：

10.1016/0378-1119(93)90553-F

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene and cDNA (TUB1) encoding an alpha-tubulin (alpha-Tub) from Pneumocystis carinii carried in rats were isolated and sequenced. The gene produced a 1.4-kb transcript that contained an open reading frame of 1347 nucleotides coding for a protein of 50 326 Da. The nt sequence of TUBI of P. carinii was 65-71% identical to other TUB1 genes, including those of yeast and human. The deduced amino acid sequence of P. carinii alpha-Tub showed 75-84% identity to other alpha-Tubs. Eight small introns resided within TUB1. P. carinii TUB1 was mapped to a 425-kb chromosome. The cloned gene appears to be the only alpha-Tub-encoding gene in the P. carinii genome.

引用

页码：137 / 141

页数：5

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