EBP-37, A NEW ELASTIN BINDING-PROTEIN IN HUMAN PLASMA - STRUCTURAL SIMILARITY TO FICOLINS, TRANSFORMING GROWTH-FACTOR-BETA-1 BINDING-PROTEINS

被引：42

作者：

HARUMIYA, S ^{[1
]}

OMORI, A ^{[1
]}

SUGIURA, T ^{[1
]}

FUKUMOTO, Y ^{[1
]}

TACHIKAWA, H ^{[1
]}

FUJIMOTO, D ^{[1
]}

机构：

[1] MITSUBISHI KASEI INST LIFE SCI,MACHIDA,TOKYO 194,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1995年 / 117卷 / 05期

关键词：

ELASTIN; ELASTIN-BINDING PROTEIN; FICOLIN; HUMAN PLASMA; TRANSFORMING GROWTH FACTOR-BETA-1;

D O I：

10.1093/oxfordjournals.jbchem.a124802

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to study the elastin-binding factors in blood, human plasma was applied to an alpha-elastin-Sepharose column. The column-binding fraction contained a 37-kDa protein, which was tentatively named EBP-37. Partial amino acid sequences of EBP-37 were determined. It had collagenous and non-collagenous domains, Homology searches of the sequences revealed that the protein is very similar but not identical to ficolins, transforming growth factor-beta 1 (TGF-beta 1)-binding proteins from porcine uterus membranes, Direct interaction of EBP-37 with elastin was confirmed by demonstrating the binding of the isolated EBP-37 to alpha-elastin on a nitrocellulose membrane using the EBP-37-specific antiserum, The existence of oligomers and multimers crosslinked by disulfide bonds was demonstrated by immunoblot analysis, Possible functions of EBP-37 are discussed.

引用

页码：1029 / 1035

页数：7

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