CHARACTERIZATION OF MAPUERA-VIRUS - STRUCTURE, PROTEINS AND NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING THE NUCLEOCAPSID PROTEIN

被引:20
作者
HENDERSON, GW
LAIRD, C
DERMOTT, E
RIMA, BK
机构
[1] QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,DIV MOLEC BIOL,BELFAST BT9 7BL,ANTRIM,NORTH IRELAND
[2] QUEENS UNIV BELFAST,DEPT MICROBIOL & IMMUNOL,BELFAST BT9 7BL,ANTRIM,NORTH IRELAND
关键词
D O I
10.1099/0022-1317-76-10-2509
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The molecular biology of Mapuera virus was studied at both the protein and nucleic acid levels. Seven virus-encoded proteins were detected in infected Vero cells. The sizes and characteristics of each of the proteins determined from various radiolabelling experiments allowed preliminary identification of the proteins as the large (L; 190 kDa), haemagglutinin neuraminidase (HN; 74 kDa), nucleocapsid (N; 66 kDa), fusion (F-0; 63 kDa), phosphoprotein (P; 49 kDa), matrix (M; 43 kDa) and non-structural (V; 35 kDa) proteins. Western blot analysis showed that the HN, N and P proteins were major antigens recognized in the mouse. A cDNA library of total virus-infected cellular mRNA was created and screening of the library resulted in the detection of cDNA sequences representing the N mRNA transcript of Mapuera virus. The N mRNA sequence determined from the clones was 1731 nt in length and contained an ORF that encoded 537 amino acids, the complete 3' untranslated region and part of the 5' non-coding region. The calculated M(r) of the N protein was 59 kDa, which is close to the 66 kDa protein observed by SDS-PAGE.
引用
收藏
页码:2509 / 2518
页数:10
相关论文
共 44 条
[31]   STRUCTURE, FUNCTION, AND INTRACELLULAR PROCESSING OF PARAMYXOVIRUS MEMBRANE-PROTEINS [J].
MORRISON, TG .
VIRUS RESEARCH, 1988, 10 (2-3) :113-135
[32]   PROTEOLYTIC CLEAVAGE OF SUBUNITS OF NUCLEOCAPSID OF PARAMYXOVIRUS SIMIAN VIRUS 5 [J].
MOUNTCASTLE, WE ;
COMPANS, RW ;
LACKLAND, H ;
CHOPPIN, PW .
JOURNAL OF VIROLOGY, 1974, 14 (05) :1253-1261
[33]   POLYPEPTIDE-SYNTHESIS IN MUMPS VIRUS-INFECTED CELLS [J].
RIMA, BK ;
ROBERTS, MW ;
MCADAM, WD ;
MARTIN, SJ .
JOURNAL OF GENERAL VIROLOGY, 1980, 46 (FEB) :501-505
[34]   THE EVOLUTION OF MORBILLIVIRUSES - A COMPARISON OF NUCLEOCAPSID GENE-SEQUENCES INCLUDING A PORPOISE MORBILLIVIRUS [J].
RIMA, BK ;
WISHAUPT, RGA ;
WELSH, MJ ;
EARLE, JAP .
VETERINARY MICROBIOLOGY, 1995, 44 (2-4) :127-134
[35]  
RIMA BK, 1995, VIRUS TAXONOMY, V10, P268
[36]   CONSERVED STRUCTURES AMONG THE NUCLEOCAPSID PROTEINS OF THE PARAMYXOVIRIDAE - COMPLETE NUCLEOTIDE-SEQUENCE OF HUMAN PARA-INFLUENZA VIRUS TYPE-3 NP MESSENGER-RNA [J].
SANCHEZ, A ;
BANERJEE, AK ;
FURUICHI, Y ;
RICHARDSON, MA .
VIROLOGY, 1986, 152 (01) :171-180
[37]   NUCLEOTIDE-SEQUENCE OF THE LEADER AND NUCLEOCAPSID PROTEIN GENE OF MUMPS-VIRUS AND EPITOPE MAPPING WITH THE INVITRO EXPRESSED NUCLEOCAPSID PROTEIN [J].
TANABAYASHI, K ;
TAKEUCHI, K ;
HISHIYAMA, M ;
YAMADA, A ;
TSURUDOME, M ;
ITO, Y ;
SUGIURA, A .
VIROLOGY, 1990, 177 (01) :124-130
[38]   2 MESSENGER-RNAS THAT DIFFER BY 2 NONTEMPLATED NUCLEOTIDES ENCODE THE AMINO COTERMINAL PROTEIN-P AND PROTEIN-V OF THE PARAMYXOVIRUS-SV5 [J].
THOMAS, SM ;
LAMB, RA ;
PATERSON, RG .
CELL, 1988, 54 (06) :891-902
[39]   MOLECULAR RELATIONSHIPS BETWEEN HUMAN PARAINFLUENZA VIRUS TYPE-2, AND SIMIAN VIRUS-41 AND VIRUS-5 - DETERMINATION OF NUCLEOPROTEIN GENE-SEQUENCES OF SIMIAN VIRUS-41 AND VIRUS-5 [J].
TSURUDOME, M ;
OKI, N ;
HIGUCHI, Y ;
MIYAHARA, K ;
YOSHIMOTO, M ;
MUTSUGA, N ;
KITADA, S ;
OGAWA, M ;
MIYAHARA, Y ;
OKAMOTO, K ;
KAWANO, M ;
KOMADA, H ;
MATSUMURA, H ;
KUSAGAWA, S ;
NISHIO, M ;
ITO, Y .
JOURNAL OF GENERAL VIROLOGY, 1991, 72 :2289-2292
[40]   EDITING OF THE SENDAI VIRUS P/C MESSENGER-RNA BY G-INSERTION OCCURS DURING MESSENGER-RNA SYNTHESIS VIA A VIRUS-ENCODED ACTIVITY [J].
VIDAL, S ;
CURRAN, J ;
KOLAKOFSKY, D .
JOURNAL OF VIROLOGY, 1990, 64 (01) :239-246