MILD PROCEDURE FOR THE RAPID RELEASE OF CYTOPLASMIC ENZYMES FROM CULTURED ANIMAL-CELLS

被引：148

作者：

MACKALL, J

MEREDITH, M

LANE, MD

机构：

[1] Department of Physiological Chemistry, The Johns Hopkins University School of Medicine, Baltimore

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90216-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid release of cytosolic enzymes from animal cells in monolayer culture is achieved by treatment of dispersed cells with digitonin. This mild procedure causes efficient cell lysis (>95%) with minimal organelle disruption. A second procedure suitable for isolation of cytosolic enzymes by direct digitonin treatment of cell monolayers has also been developed. © 1979.

引用

页码：270 / 274

页数：5

共 15 条

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