ARTOCARPIN GALACTOMANNAN INTERACTION - CHARACTERIZATION OF COMBINING SITE OF ARTOCARPIN

被引：9

作者：

CHOWDHURY, S ^{[1
]}

CHATTERJEE, BP ^{[1
]}

机构：

[1] INDIAN ASSOC CULTIVAT SCI,DEPT BIOL CHEM,CALCUTTA 700032,W BENGAL,INDIA

来源：

PHYTOCHEMISTRY | 1993年 / 32卷 / 02期

关键词：

ARTOCARPUS-LAKOOCHA; MORACEAE; SEEDS; ARTOCARPIN; LECTIN; GALACTOMANNAN; COMBINING SITES; INTERACTION;

D O I：

10.1016/S0031-9422(00)94975-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Artocarpin, a lectin purified from Artocarpus lakoocha seeds, precipitates well several galactomannans having terminal D-Gal-alpha-(1-6)-residues. Quantitative precipitin-inhibition studies using various haptens suggest that the -OCH2- group at C-1 and hydroxyl groups at C-4, and partially at C-6, in the alpha-glycoside of D-galactose configuration are necessary for lectin-sugar interaction. The formation of an artocarpin-fenugreek galactomannan complex was dependent on temperature, pH, ionic strength and the number of hydroxy groups of alditols added to the medium.

引用

页码：243 / 249

页数：7

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