ONTOGENY OF ALPHA-1-ISOFORMS AND BETA-1-ISOFORMS OF NA+-K+-ATPASE IN FETAL DISTAL RAT LUNG EPITHELIUM

被引:55
作者
OBRODOVICH, H
STAUB, O
ROSSIER, BC
GEERING, K
KRAEHENBUHL, JP
机构
[1] SWISS INST EXPTL CANC RES, CH-1066 EPALINGES, SWITZERLAND
[2] UNIV LAUSANNE, INST PHARMACOL & TOXICOL, CH-1005 LAUSANNE, SWITZERLAND
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 05期
关键词
ALVEOLAR EPITHELIUM; TYPE-II PNEUMONOCYTES; SODIUM TRANSPORT; LUNG DEVELOPMENT; SODIUM-POTASSIUM ADENOSINE-TRIPHOSPHATASE;
D O I
10.1152/ajpcell.1993.264.5.C1137
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Because immature, in contrast to mature, fetal lungs have ineffective Na transport, we wished to determine the ontogeny of Na+-K+-ATPase expression in fetal distal lung epithelium (FDLE). FDLE and fibroblasts (FLF) from 17- to 22-day gestational age fetal rats (term = 22 days) were grown in primary culture. Northern and slot-blot analyses utilizing isoform-specific cDNA probes determined that alpha1- (3.7 kb) and beta1- (2.7,2.3, and 1.9 kb) transcripts were present in FDLE at levels approximately fivefold higher than in FLF. Alpha2-, alpha3-, or beta2-isoforms of Na+-K+-ATPase were not detected. In 17-day gestational age FDLE, only small amounts of alpha1-mRNA levels were detectable, and there were approximately 10-fold less beta1-isoform transcripts. By 20 days gestational age, the level of alpha1-transcripts roughly doubled, whereas beta1-levels increased approximately sixfold. Thus, during the transition from the canalicular to saccular stages of lung development, FDLE have a differentially regulated surge in mRNA levels of alpha1- and beta1-Na+-K+-ATPase isoforms and do not switch isoforms during lung development. Levels for both isoform transcripts then fell before birth, reaching values less than those seen for 17-day gestational age FDLE. FDLE vesicle Na+-K+-ATPase activity did not increase until 22 days gestational age.
引用
收藏
页码:C1137 / C1143
页数:7
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