MEASUREMENT OF PHOSPHOLIPID MONOLAYER SURFACE-POTENTIALS AT A HYDROCARBON-ELECTROLYTE INTERFACE

被引:13
作者
PICKARD, WF [1 ]
SEHGAL, KC [1 ]
JACKSON, CM [1 ]
机构
[1] WASHINGTON UNIV,DEPT BIOL CHEM,ST LOUIS,MO 63130
关键词
(Phospholipid monolayer); Hydrocarbon-electrolyte interface; Surface potential; Surface pressure;
D O I
10.1016/0005-2736(79)90241-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The phospholipid monolayer spread at a hydrocarbon-electrolyte interface can be used as a model system for the plasma membrane and its properties and structure probed by measurements of surface pressure and surface potential. To facilitate such studies, (i) the theory of the vibrating plate (Kelvin) method of measuring surface potentials is reëxamined and a new interpretation given for the potentials measured and (ii) a new apparatus for performing these measurements is described. The theory and apparatus are illustrated by measurements on films of distearoyl phosphatidylcholine at the interface between 2,2,4-trimethylpentane (isooctane) and 0.1 M NaCl. © 1979.
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页码:1 / 10
页数:10
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