INTERRELATIONSHIP OF SOMATOSTATIN, INSULIN, AND CALCIUM IN THE CONTROL OF GLUCONEOGENESIS IN KIDNEY CORTEX SLICES

The effects of somatostatin and insulin on renal gluconeogenesis and the involvement of Ca++ in somatostatin action were investigated. Addition of somatostatin stimulated gluconeogenesis in the renal cortex and this stimulation was inhibited by insulin. Maximum stimulatory effect by somatostatin was observed at 30 minutes. No stimulatory effect of somatostatin was observed in calcium depleted kidney cortex slices. However, addition of Ca++ completely restored the stimulatory effect of somatostatin. These results suggest that insulin and somatostatin may be playing important physiological roles in renal gluconeogenesis and that Ca++ is required for the somatostatin-induced enhancement in gluconeogenic activity. © 1979.