AGE-DEPENDENT CHANGES OF NO SYNTHASE ACTIVITY IN THE RAT-BRAIN

被引:49
作者
MOLLACE, V [1 ]
RODINO, P [1 ]
MASSOUD, R [1 ]
ROTIROTI, D [1 ]
NISTICO, G [1 ]
机构
[1] UNIV REGGIO CALABRIA,FAC PHARM,CNR,IBAF CTR,CATANZARO,ITALY
关键词
D O I
10.1006/bbrc.1995.2537
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report that NO synthase activity, as expressed by citrulline and nitrite formation in brain homogenates, is decreased in 24-month old in comparison to 3-month old rats. In particular, a Ca++-dependent NO synthase activity was detected in homogenates obtained from cortical, hippocampal, cerebellar and lower brain stem slices from both 3- and 24 month - old rats. The amount of citrulline generated from L-arginine was significantly decreased in the hippocampus and lower brain stem by 40 and 48%, respectively. No changes were observed in NO synthase activity in cortical and cerebellar homogenates. Thus, the L-arginine-NO pathway seems to be impaired in selected areas of rat brain and this may contribute to the understanding of pathophysiological mechanisms underlying age-related cerebral disorders. (C) 1995 Academic Press. Inc.
引用
收藏
页码:822 / 827
页数:6
相关论文
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