ORIGIN OF THE NITROGEN ATOM OF PYRIDOXINE IN SACCHAROMYCES-CEREVISIAE

被引：35

作者：

TAZUYA, K

ADACHI, Y

MASUDA, K

YAMADA, K

KUMAOKA, H

机构：

[1] Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, 663, 11-68, Koshien Kyuban-cho

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1244卷 / 01期

关键词：

PYRIDOXINE; BIOSYNTHESIS; GLUTAMINE; (SACCHAROMYCES-CEREVISIAE);

D O I：

10.1016/0304-4165(94)00205-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The origin of the nitrogen atom of pyridoxine was studied in Saccharomyces cerevisiae. The N-15 atom of (NH4Cl)-N-15 added to the growth medium as the nitrogen source was incorporated efficiently into the nitrogen atom of pyridoxine. The competitive effects of N-14-amino acids on the incorporation of (NH4Cl)-N-15 were examined. Incorporation of N-15 into pyridoxine was inhibited by glutamine. The label of L-[amide-N-15]glutamine was incorporated effectively into pyridoxine in S. cerevisiae. On the other hand, the label from L-[amide-N-15]glutamine was not incorporated into pyridoxine in Escherichia coli. These findings suggest that the biosynthetic pathway of pyridoxine in S. cerevisiae differs from that in E. coli.

引用

页码：113 / 116

页数：4