TRANSFORMING GROWTH-FACTOR-BETA-1 (TGF-BETA-1) INHIBITS DNA-SYNTHESIS OF PWM-STIMULATED PBMC VIA SUPPRESSION OF IL-2 AND IL-6 PRODUCTION

被引：46

作者：

REINHOLD, D ^{[1
]}

BANK, U ^{[1
]}

BUHLING, F ^{[1
]}

LENDECKEL, U ^{[1
]}

ULMER, AJ ^{[1
]}

FLAD, HD ^{[1
]}

ANSORGE, S ^{[1
]}

机构：

[1] FORSCHUNGSINST BORSTEL,DEPT IMMUNOL & CELL BIOL,W-2061 BORSTEL,GERMANY

来源：

CYTOKINE | 1994年 / 6卷 / 04期

关键词：

CYTOKINE PRODUCTION; DNA SYNTHESIS; PWM-STIMULATED PBMC; TGF-BETA-1;

D O I：

10.1016/1043-4666(94)90062-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The multifunctional cytokine transforming growth factor-β1 (TGF-β1) is known to inhibit the proliferation of lymphocytes. However, the role of TGF-β1 in the production and secretion of various interleukins is not yet clear. In this study we have analysed in parallel the effects of TGF-β1 on both DNA synthesis and production of the cytokines IL-1, IL-2, IL-6, and TNF-α by pokeweed mitogen-stimulated peripheral blood mononuclear cells. With this stimulation system we show that TGF-β1 at a concentration of 15 ng/ml significantly suppresses IL-2 and IL-6 production. The release of IL-1 and TNF-α, however, was not influenced under these conditions. Under similar conditions DNA synthesis of PWM-stimulated PBMC was found to be inhibited by 50 ± 10%. Using flow cytometric methods we could demonstrate that TGF-β1 arrested the cells in the G0/G1 phase of the cell cycle. Taken together, these results suggest that TGF-β1 may suppress immune responses by inhibiting the endogenous production of IL-2 and IL-6. © 1994 Academic Press Limited.

引用

页码：382 / 388

页数：7